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Automated Hemagglutination Analyzer
Cypher One is a new tool for rapid automated analysis of hemagglutination (HA) and hemagglutination inhibition (HI) assays. Until now interpretation of HA and HI assays required specialized expertise to accurately read and manually record the data, with experienced users often differing in their interpretation of the titer value. The Cypher One system will not only standardize analysis but also provide a digital, traceable record to enhance data quality and reliability.
“The system is really ingenious, easy to use….” Scientist, Vaccine Industry
“The Cypher One automated hemagglutination analyzer can eliminate subjectivity within an institution and has the potential to standardize interpretation of HA and HI assays across the vaccine industry. Having trained others to interpret the HA assay I understand that subjective interpretation is a major concern.” Vice President of R&D, Vaccine Industry
“We are very satisfied with the results from Cypher One; it was able to determine titer values very similar to the manual interpretations determined by our lab.” Scientist, Vaccine Industry
“Correlation with my manual read is quite good within +/- 1 dilution well.” Associate Professor, Infectious Diseases
Cypher One Advantages:
- Traceable digital record of each plate image, titer determinations, sample identification, and associated experimental parameters
- Proprietary image analysis algorithm provides standardized interpretation of HA/HI results
- Improved accuracy and reproducibility by eliminating subjectivity of plate analysis and data tracking errors
- Streamlined workflow and reduced burden on operators
Cypher One Features:
- Rapid, non-subjective, standardized analysis of HA and HI assays
- Plates imaged and analyzed within 1 minute
- Each image and corresponding interpretation are permanently linked and saved to a database as a digital record for long term storage and reference
- High resolution image allows the algorithm to accurately differentiate between agglutinated and non-agglutinated wells without tilting, even in instances of incomplete button formation
- Strong correlation with manual (“by eye”) analysis
- Two user-controlled analysis parameters for optimization across varying conditions (e.g. different blood types, blood concentrations, etc.)
- Batch processing feature
- Easily created templates to quickly and easily auto-populate conserved experimental parameters
- Plates available from InDevR that meet specifications for the Cypher One Instrument
Cypher One Applications:
- Characterization of viruses, bacteria and antibodies
- Determining immunogenicity of vaccines (e.g., Influenza, HIV, Ebola)
- Antigenic characterization of new influenza viruses
Download Application and Technical Notes:
Cypher One Tips & Techniques
- Assay Setup:
- Not all 96 well microplates are compatible with the Cypher One receiver plate. We strongly recommend purchasing plates from InDevR to ensure compatibility with Cypher One. Both round (U) and conical (V) bottom are available.
- For information regarding HA and HI assays and recommended protocols, please refer to the CDC’s Influenza Antigenic Characterization page or the CONSISE website.
- The Cypher One software is designed to analyze samples that have been serially diluted across a single row (i.e. A1-A11) with the control wells in the last column (i.e. A12, B12, etc.). For more information about the assay setup, please refer to Cypher One Technical Note #1.
- If any bubbles are present in the assay, pop them prior to imaging so they do not interfere with the image analysis.
- Imaging & Analysis with the Cypher One Software:
- Do not tilt the plate prior to imaging.
- Orient the plate so that the A1 well of the microplate is aligned with the A1 sticker on the receiver plate.
- The Cypher One tray can be completely removed from the instrument for decontamination in case of spillage.
- Be sure the instrument is turned on before imaging. The power button on the instrument will illuminate to indicate it is turned on.
- Experiment Settings and Templates:
- Be sure to select the correct assay type (HA or HI) in the experiment template section.
- In order to create a new experimental template, create a copy of an existing template and enter a new template name and appropriate experimental settings. Select save to create the new template.
- Be very descriptive when naming experiment templates so they can be easily recalled when necessary. For example, include the blood type, blood concentration, plate type and virus type.
- In a high throughput environment, plate names may be automatically appended with a consecutive number.
- Analysis Settings and Templates:
- When tuning your plate, be sure to select “Show Tuning Info”. First adjust the “Filter” value by increasing until the majority of the fully agglutinated well show a tuning value greater than 0, then decrease the filter value one step. Then adjust the “Cutoff” value until the appropriate wells are highlighted. For more information on tuning, please refer to the operation manual.
- Be very descriptive when naming analysis templates so they can be easily recalled when necessary. For example, include the blood type, blood concentration, plate type and virus type.
- Accurate pipetting is critical in being able to apply a saved analysis template across plates with conserved experimental parameters (blood type, concentration, plate type, virus).
- Plates can be “closed” from the database to hide from view. In order for these plates to become visible again, the software must be closed and re-opened.
- The power settings for the laptop should not be changed as this is the primary control for turning off the instrument’s light source when the instrument is in between use sessions. Although this feature exists, it is important to turn the instrument off on a daily basis.
- Results from a plate analysis as well as the image may be printed as a screen capture by selecting the Print action button in the View Plate mode.
- One or many results from analyzed plates may be exported as a CSV file by using the Ctrl/Shift keys within the Database View.
- Once a plate analysis has been tuned and satisfied results are acquired, the analysis may be approved by selecting the Approve action button in the View Plate mode. This will create a read-only copy. Approved plates can only be edited if a copy is created within the View Plate mode.